Genetically Engineered Mouse Core
(Advanced Technology Core Laboratories, Dan L. Duncan Cancer Center, Lester and Sue Smith Breast Center)
Baylor College of Medicine
Michael E. DeBakey Center (BCM)
One Baylor Plaza
BCM-Michael DeBakey Center, Room: M725A
Houston, Texas 77030
- Director: Francesco DeMayo (713) 798-6241
The mission of the Genetically Engineered Mouse (GEM) Shared Resource is to aid Cancer Center Investigators with the design and execution of experiments, to utilize animal models for the investigation of the progression of cancer, and to conduct preclinical evaluation of therapies for the treatment of cancer.
- Charge for services or equipment: Yes
- Equipment Training: No
- Methodology Training: No
Generation of Transgenic Mice by the Microinjection of DNA
This service will include: Injection of traditional DNA constructs, into the pronucleus of the one-cell mouse embryo and the generation of mice. Injection of BAC DNA constructs , into the pronucleus of the one-cell mouse embryo and the generation of mice. Culture of one cell mouse embryos with Lentivirus' containing transgenes and the generation of mice.
The GEM Shared Resource will aid Cancer Center Investigators by the cryopreservation and rederivation of strains of genetically engineered animal models.
These services will include Embryo cryopreservation, recovery for storage and embryo transfer to regenerate genetically engineered mouse lines. Sperm Cryopreservation, recovery from storage, 1n vitro fertilization and and embryo transfer to regenerate genetically engineered mouse lines. Embryo transfer from imported strains into SPF facility.
The GEM Shared Resource will coordinate the production of animals for experimental analysis for Investigators.
This service will include: The combining superovulation and embryo transfer to rapidly provide Investigators with mice needed for experimental studies.
The GEM Shared Resource will generate mutant mice by the culture and manipulation of Embryonic Stem Cells.
This service will include: ES cell electroporation of DNA into ES cells. ES cell clone expansion. Generation of primary ES cells ES cell microinjection into blastocyst and the generation of chimeric mice. The generation of ES cells from recombinant mouse strains.